Shoot apex transformation - - claims in plain english - patent granted to The Texas A & M University System
Specific Patent Information
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Patent Number
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Title, Independent Claims and Summary of Claims
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Assignee
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US
5164310
- Earliest priority - 1 June 1988
- Filed - 5 February 1991
- Granted - 17 November 1992
- Expected expiry - 5 February 2011
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Title - Method for transforming plants via the shoot apex
Claim 1
A method of transforming excised shoot apical tissue comprising:
a) excising shoot apical tissue consisting essential of the apical dome and
two or more primordial leaves,
b) placing said excised tissue in a suitable growth medium,
c) inoculating said apical tissue with Agrobacterium tumefaciens to
transform said tissue.
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Claim 8
A method for transforming an excised shoot apex comprising:
a) excising a shoot apex,
b) placing said apex in a suitable growth
medium,
c) inoculating said apical tissue with Agrobacterium
tumefaciens to transform said tissue.
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The United States patent 5 164 310 claims a method to transform shoot apices,
which contain the apical dome with meristematic tissue and some primordial
leaves, with A. tumefaciens. According to the inventors, shoot cultures
develop roots directly and rapidly, and plant regeneration is achieved within
six weeks after transformation.
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Texas A&M Univeristy System
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| Remarks |
- National phase entry of WO 89/12102 in Australia (AU 3756889) has lapsed on
26 April 1991.
- Related patent application filed in China (CN 1042638) is deemed withdrawn
on 1 April 1992.
- National phase entry of WO 89/12102 in Europe (EP 419533) is deemed
withdrawn on 10 April 1993.
- National phase entry of WO 89/12102 in Japan (JP 2996995) has been granted
on 29 October 1999.
- Other related patent applications filed in Denmark (DK 285590), Spain (ES
2017024, reported on INPADOC as lapsed), Ireland (IE 65516), Israel (IL 90440),
South Africa (ZA 8904379).
- Related patent in New Zealand (NZ 229340) has been granted and published as
registered on 26 May 2000.
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