In planta transformation - patent granted to Cotton Inc
Specific Patent Information
|
Patent Number
|
Title, Independent Claims and Summary of Claims
|
Assignee
|
|
US
5994624
- Earliest priority - 20 October 1997
- Filed - 20 October 1997
- Granted - 30 November 1999
- Expected expiry - 20 October 2017
|
Title - In planta method for the production of transgenic
plants
Claim 1
A method for producing a transformed plant comprising, injecting
Agrobacterium cells harboring a vector, comprising a nucleic acid
molecule capable of conferring a desired phenotypic trait to a plant, into a
plant floral or meristematic tissue using a needleless injection device, which
can be adapted for the injection of small volumes of material in a precise
manner without causing massive tissue damage.
|
Claim 16
A method for producing a transgenic seed comprising injecting a
Agrobacterium cells harboring a vector, comprising a gene capable of
conferring a desired phenotypic trait, into the floral tissues of a plant before
the division of the egg cell using a needleless-hypodermic injection device.
|
Claim 17
A method of producing a transgenic seed comprising injecting a recombinant
Agrobacterium into the foloral tissues of a plant using a
needleless-hypodermic injection device.
|
Claim 18
A method for producing a transgenic seed comprising injecting a
Agrobacterium cells harboring a vector, comprising a gene capable of
conferring a desired phenotypic trait, into the floral tissues of a plant before
the division of the egg cell using a needleless-hypodermic injection device.
|
The United States patent 5 994 624 discloses
- a method to transform floral or meristematic tissue in vivo by
injecting with Agrobacterium with a needleless hypodermic injection
device; and
- a method to transform floral tissue before the division of the egg cell
using the same procedure as above.
The transformed floral tissue develops normally, forming seeds after
pollination. Seeds are grown to generate a F1 transformed progeny.
Although claim 1 says that the injection device can
be adapted for the injection of small volumes of material in a
precise manner without causing massive tissue damage, it does not have
to be. The specification, further, does not explicitly define what
"massive tissue damage" is though it discloses that direct injection with a
needle or particle bombardment can cause such damage.
|
Cotton Inc.
|
|
AU
752717
- Earliest priority - 20 October 1997
- Filed - 19 October 1998
- Granted - 26 September 2002
- Patent reported as ceased - 27 May 2004
|
Title - In planta method for the production of
transgenic plants
Claim 1
A method for producing a transformed plant including injecting a transforming
agent comprising Agrobacterium cells harboring a vector having a
nucleic acid molecule capable of conferring a desired phenotypic trait to a
plant, into a plant floral or meristematic tissue using a needleless injection
device which can be adapted for the injection of small volumes of material in a
precise manner without causing massive tissue damage.
|
Claim 12
A method for producing a transgenic seed comprising injecting a recombinant
Agrobacterium into the floral tissues of a plant using a needleless
injection device which can be adapted for the injection of small volumes of
material in a precise manner without causing massive tissue damage.
|
Claim 13
A method for producing a transgenic seed comprising injecting
Agrobacterium cells harboring a vector, comprising a gene capable of
conferring a desired phenotypic trait, into the floral tissues of a plant before
the division of the egg cell using a needleless injection device.
|
Claim 14
An in planta germline transformation method including needleless
injection of a transforming agent comprising an Agrobacterium vector,
said injection being directly into a tissue including the floral or meristematic
germline cells of said plant, said injection being selected as to pressure,
volume and trajectory so as to limit penetration of said transforming agent to a
region of said tissue whereby said germline cells are transformed without
causing excessive tissue damage.
|
Claim 15
A method for producing a transformed plant or a transgenic seed substantially
as herein described in the detailed description of the invention with reference
to the drawings.
|
Unlike the US patent, the claims in the AU patent do not require that the
needleless injection device be a hypodermic device.
As in the US patent, although claim 1 and 12 say that the needleless
injection device can be adapted for the injection of small volumes of material
in a precise manner without causing massive tissue damage, the claims don't
require the injection device to have these features/functions.
The patent does not disclose what "excessive" tissue damage is other than to
generally describe that it may occur through using a needle to inject a tissue.
The patent does not explicitly state that "excessive" tissue damage and
"massive" tissue damage are the same.
|
| Remarks |
Related patent application in South Africa (ZA 9809517).
|
Note: Patent information on this page was last updated on 22 February 2006.
The information contained in this page was believed to be correct at the
time it was collated. New patents and patent applications, altered
status of patents, and case law may have resulted in changes in the
landscape. CAMBIA makes no warranty that it is correct or up to date at
this time and accepts no liability for any use that might be made of it.
Corrections or updates to the information are welcome. Please send an email to
info@bios.net.
There are no comments.