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EP
1171621 B1
- Earliest priority - 19 April 1999
- Filed - 19 April 2000
- Granted - 7 December 2005
- Expected expiry - 19 April 2020
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Title - Plant transformation method
Claim 1
A transformation method comprising inoculation and co-cultivation of a target
tissue, from a target plant, with Agrobacterium, at a time when the
target tissue is in its natural plant environment, followed by generation of a
transgenic plant via dedifferentiation and regeneration of the target tissue,
wherein said inoculation is performed by injection of Agrobacterium
into said tissue, with minimal damages to said tissue.
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Claim 3
A transformation method comprising inoculation and co-cultivation of a target
tissue, from a target plant, with Agrobacterium, at a time when the
target tissue is in its natural plant environment, followed by generation of
dedifferentiated tissue from the target tissue, wherein said inoculation is
performed by injection of Agrobacterium into said tissue, with minimal
damages to said tissue.
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Claim 16
Use of Agrobacterium in a transformation method comprising
inoculation and co-cultivation of a target tissue, from a target plant, with
Agrobacterium, at a time when the target tissue is in its natural plant
environment, followed by generation of transgenic plant material via
dedifferentiation of the target tissue, wherein said inoculation is performed by
injection of Agrobacterium into said tissue, with minimal damages to
said tissue.
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The patent does not describe what consitutes "minimal damage" to a tissue
Designated States at the time of grant are: Austria, Belgium, Switzerland,
Cyprus, Germany, Denmark, Spain, Finland, France, United Kingdom, Greece,
Ireland, Italy, Liechtenstein, Luxembourg, Monaco, Netherlands, Portugal,
Sweden.
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RhoBio
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AU
775949 B2
- Earliest priority - 19 April 1999
- Filed - 19 April 2000
- Granted - 19 August 2004
- Expected expiry - 19 April 2020
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Title - Plant transformation method
Claim 1
A transformation method comprising inoculation and co-cultivation of a target
tissue, from a target plant, with Agrobacterium, at a time when the
target tissue is in its natural plant environment, followed by generation of a
transgenic plant via dedifferentiation and regeneration of the target tissue,
after isolation and culture of said target tissue, wherein said inolucation is
performed by injection of Agrobacterium into said tissue, with minimal
damage to said tissue.
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Claim 3
A transformation method comprising inoculation and co-cultivation of a target
tissue, from a target plant, with Agrobacterium, at a time when the
target tissue is in its natural plant environment, followed by generation of
dedifferentiated tissue from the target tissue after isolation and culture of
said target tissue, wherein said inoculation is performed by injection of
Agrobacterium into said tissue, with minimal damage to said tissue.
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Claim 15
Use of Agrobacterium in a transformation method comprising
inoculation and co-cultivation of a target tissue, from a target plant, with
Agrobacterium, at a time when the target tissue is in its natural plant
environment, followed by generation of transgenic plant material via
dedifferentiation of the target tissue, after isolation and culture of said
target tissue, wherein said inoculation is performed by injection of
Agrobacterium into said tissue, with minimal damage to said tissue.
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WO
2000/63398
- Earliest priority - 19 April 1999
- Filed - 19 April 2000
- OPI - 16 October 2000
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Title - Plant transformation method
Claim 1
A transformation method comprising inoculation and co-cultivation of a target
tissue, from a target plant, with Agrobacterium, at a time when the
target tissue is in its natural plant environment, followed by generation of a
transgenic plant via dedifferentiation and regeneration of the target tissue.
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Claim 3
A transformation method comprising inoculation and co-cultivation of a target
tissue, from a target plant, with Agrobacterium, at a time when the
target tissue is in its natural environment, followed by generation of
dedifferentiated tissue from the target tissue.
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Claim 14
Use of Agrobacterium in a transformation method comprising
inoculation and co-cultivation of a target tissue, from a target plant, with
Agrobacterium, at a time when the target tissue is in its natural
environment, followed by generation of transgenic plant material via
dedifferentiation of the target tissue.
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The PCT application WO 0063398 (equivalent to the European
application EP 1 171 621 A1) includes claims to:
- a method to transform a plant tissue in vivo by inoculating and
co-cultivating Agrobacterium cells with the tissue. The transformed
tissue is separated from the plant and submitted to a dedifferentiation and
regeneration process.
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