Patent application filed by Japan Tobacco Inc.
The European application filed by Japan Tobacco discloses
methods for co-transforming plants with two T-DNAs. One of the T-DNAs has a gene
conferring drug-resistance and the other has a gene of interest and is part of a
hybrid plasmid. The hybrid plasmid is constructed by homologous recombination of
an acceptor and an intermediate plasmid.
Specific Patent Information
|
Patent Number
|
Title, Independent Claims and Summary of Claims
|
Assignee
|
EP 687730 A1
- Earliest priority - 8 December 1993
- Filed - 6 December 1994
- Application pending
|
Title - Method of transforming plant and vector therefor
Claim 1
A method for transforming a plant through a bacterium belonging to the genus
Agrobacterium, comprising:
A) co-transforming plant cells with a first T-DNA (1) and a second T-DNA (2);
and
B) selecting the cells which acquired drug resistance; said first T-DNA
(1) containing a gene giving said drug resistance, which function in said plant;
said second T-DNA (2) containing a desire DNA fragment to be introduced into
said plant, the second T-DNA (2) being contained in a hybrid vector;
said
hybrid vector being prepared by homologous recombination between an acceptor
vector and an intermediate vector in said bacterium belonging to genus
Agrobacterium;
said acceptor vector containing at least:
(i) a DNA
region having a function to replicate a plasmid in said bacterium belonging to
the genus Agrobacterium and Escherichia coli,
(ii) a DNA region containing
vir B gene and vir G gene in virulence region of Ti plasmid pTiBo542 of
Agrobacterium tumefaciens, and
(iii) a DNA region which is homologous with
a part of said intermediate vector, which is subjected to homologous
recombination in said bacterium belonging to genus Agrobacterium;
said
intermediate vector containing at least:
(i) a DNA region having a function
to replicate a plasmid in Escherichia coli, which does not function in said
bacterium belonging to genus Agrobacterium,
(ii) a DNA region which is
homologous with a part of said acceptor vector, which is subjected to homologous
recombination in said bacterium belonging to genus Agrobacterium, and
(iii) a DNA region which constitutes at least a part of said second T-DNA.
|
Claim 16
A hybrid vector comprising a first T-DNA containing: 1) a gene giving a drug
resistance, which functions in plant, and
2) a second T-DNA giving a restriction site; said hybrid vector being
prepared by homologous recombination between an acceptor vector and an
intermediate vector in a bacterium belonging to genus Agrobacterium;
said acceptor vector containing at least:
(i) a DNA region having a
function to replicate a plasmid in said bacterium belonging to the genus
Agrobacterium and Escherichia coli,
(ii) a DNA region containing vir B
gene and vir G gene in virulence region of Ti plasmid pTiBo542 of Agrobacterium
tumefaciens, and
(iii) a DNA region which is homologous with a part of said
intermediate vector, which is subjected to homologous recombination in said
bacterium belonging to genus Agrobacterium;
said intermediate vector
containing at least:
(i) a DNA region having a function to replicate a
plasmid in Escherichia coli, which does not function in said bacterium belonging
to genus Agrobacterium,
(ii) a DNA region which is homologous with a part
of said acceptor vector, which is subjected to homologous recombination in said
bacterium belonging to genus Agrobacterium, and
(iii) a DNA region which
constitutes at least a part of said second T-DNA.
|
The claims of the application EP 687 730 A1 recite the
transformation of a plant with two different T-DNAs via Agrobacterium.
The first T-DNA contains a gene conferring drug resistance to the plant and the
second one contains a DNA fragment of interest. In the method claim (claim 1),
the second T-DNA is part of a hybrid vector formed by homologous recombination
between an acceptor vector and an intermediate vector.
A hybrid vector (claim 16) harboring the first T-DNA as described and a
second T-DNA with a cloning site is also formed by homologous recombination of
the vectors mentioned above.
Elements of the acceptor vector and the intermediate vector are also recited
in the claims.
|
Japan Tobacco Inc.
|
Note: Patent information on this page was last updated on 2 March 2006.
The information contained in this page was believed to be correct at the
time it was collated. New patents and patent applications, altered
status of patents, and case law may have resulted in changes in the
landscape. CAMBIA makes no warranty that it is correct or up to date at
this time and accepts no liability for any use that might be made of it.
Corrections or updates to the information are welcome. Please send an email to
info@bios.net.
There are no comments.