Tomato

A transformed Lycopersicon esculentum cotyledon cell, wherein said cell is present in an in vitro cell culture.

A method for transforming tomato species cells, said method comprising:

A) pre-incubating tomato cotyledon sections with medium conditioned by a plant cell feeder culture;
B) co-cultivating said cotyledon sections with Agrobacterium tumefaciens cells comprising vir genes, wherein said Agrobacterium cells further comprise DNA construct comprising:
(i) transcriptional initiation and termination regulatory regions functional in tomato plant cells and
(ii) a gene other than the wild-type gene associated with at least one of said transcriptional initiation and termination regions, and
(iii) at least a right T-DNA border, whereby said construct becomes integrated into the genome of cells in said cotyledon section to provide transformed tomato plant cells; C) incubating said transformed tomato plant cells in a regeneration medium comprising a bacteriocide and a means for selection of said transformed tomato plant cells as the result of a marker on said DNA construct, whereby transformed tomato shoots develop; and
D) transferring said transformed shoots to a rooting medium to produce transformed tomato plants.

A method for modifying the genotype of tomato plant cells, said method comprising:

A) pre-incubating tomato cotyledon sections with medium conditioned by a plant cell feeder culture,
B) contacting said cotyledon sections with a culture of a disarmed Agrobacterium tumefaciens strain comprising vir genes and a binary vector plasmid comprising at least the right T-DNA border and a gene of interest, wherein said gene of interest is under regulatory control of transcriptional initiation and termination regions functional in tomato plant cells, and wherein said gene of interest is integrated into the genome of cells in said tomato cotyledon sections, and
C) isolating said tomato cells comprising said integrated gene of interest.

A method for transforming tomato species cells, said method comprising:

A) co-cultivating tomato cotyledon sections with Agrobacterium tumefaciens cells comprising vir genes, wherein said Agrobacterium cells further comprise a DNA construct comprising transcriptional initiation and termination regulatory regions functional in tomato plant cells and a gene other than the wild-type gene associated with at least one of said transcriptional initiation and termination regions, and at least a right T-DNA border, whereby said construct becomes integrated into the genome of cells in said cotyledon section to provide transformed tomato plant cells;
B) incubating said transformed tomato plant cells in a regeneration medium comprising a bacteriocide and a means for selection of said transformed tomato plant cells as the result of a marker on said DNA construct, whereby transformed tomato shoots develop; and
C) transferring said transformed shoots to a rooting medium to produce transformed tomato plants.

A method for modifying the genotype of tomato plant cells, said method comprising:

A) contacting tomato cotyledon sections with a culture of a disarmed Agrobacterium tumefaciens strain comprising vir genes and a binary vector plasmid comprising at least the right T-DNA border and a gene of interest, wherein said gene of interest is under regulatory control of transcriptional initiation and termination regions functional in tomato plant cells, and wherein said gene of interest is integrated into the genome of cells in said tomato cotyledon sections, and
B) isolating said tomato cells comprising said integrated gene of interest.