Patent granted to Washington University
The present United States patent granted to Washington
University discloses the transformation of dicot plants with an
Agrobacterium vector having the cytokinin gene of the T-DNA region
inactivated. According to the USPTO assignments database, this patent was
exclusively licensed to
Syngenta.
In a wildtype T-DNA of a Ti plasmid, the genes encoding phytohormones are
responsible for the tumorous state of a transformed tissue. Cytokinin, one of
those phytohormones, induces the formation of shoots in a tumor.
In the disclosed invention, regeneration of a transformed dicot plant is
achieved by inactivating the cytokinin gene. Additionally, the cytokinin gene is
replaced by foreign DNA. The plasmid containing the mutant T-DNA, with foreign
DNA replacing the cytokinin gene, is accomplished by homologous recombination
within Agrobacterium.
Specific Patent Information
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Patent Number
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Title, Independent Claims and Summary of Claims
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Assignee
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US
6051757
- Earliest priority - 4 November 1983
- Filed - 5 June 1995
- Granted - 18 April 2000
- Expected expiry - 17 April 2017
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Title - Regeneration of plants containing genetically
engineered T-DNA
| Claim 1
A method of transforming a dicotyledonous plant susceptible to transformation
by Agrobacterium, comprising:
contacting the plant with an Agrobacterium tumefaciens bacterium
comprising a gene vector, the vector comprising
(i) DNA foreign to the
Agrobacterium, and
(ii) the vector not comprising a functional
cytokinin autonomy gene.
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| Claim 2
A method for producing a morphologically and developmentally normal
dicotyledonous plant comprising non-Agrobacterium foreign DNA stably
integrated in the plant's genome, said method comprising the following steps:
A) transforming a dicotyledonous plant cell susceptible to transformation by
Agrobacterium with an Agrobacterium -derived gene vector, said
vector comprising
(i) non-Agrobacterium foreign DNA and
(ii)
the vector not comprising a functional cytokinin autonomy gene; and
B) regenerating said transformed plant cell to produce a morphologically and
developmentally normal transformed plant with said foreign DNA stably integrated
in the plant's genome.
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| Claim 5
A method for producing a transgenic dicotyledonous plant comprising a stably
integrated non-Agrobacterium foreign DNA, the method comprising:
A) sexually propagating a dicotyledonous plant comprising
non-Agrobacterium foreign DNA stably integrated into its genome, said
foreign DNA having been introduced into the genome by an
Agrobacterium-derived gene vector not comprising a functional
cytokinin-autonomy gene; and
B) selecting for progeny plants which comprise
the non-Agrobacterium foreign DNA stably integrated into the genome of
said progeny plants.
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| Claim 6
A method for producing a transgenic dicotyledonous plant comprising stably
integrated non-Agrobacterium foreign DNA, the method comprising:
A) sexually propagating a dicotyledonous plant comprising
non-Agrobacterium foreign DNA stably integrated into its genome, said
plant derived from a dicotyledonous plant which was transformed by
Agrobacterium-mediated transformation with a gene vector comprising
(i) said non-Agrobacterium foreign DNA and
(ii) not
comprising a functional cytokinin autonomy gene; and
B) obtaining a progeny plant which comprises the non-Agrobacterium
foreign DNA stably integrated into its genome.
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| Claim 7
A method for producing a transgenic dicotyledonous plant comprising stably
integrated non-Agrobacterium foreign DNA, the method comprising:
A) propagating a dicotyledonous plant comprising non-Agrobacterium
foreign DNA stably integrated into its genome, said plant derived from a
dicotyledonous plant which was transformed by Agrobacterium-mediated
transformation with a gene vector comprising
(i) said
non-Agrobacterium foreign DNA and
(ii) not comprising a functional
cytokinin autonomy gene; and
B) obtaining a plant which comprises the non-Agrobacterium foreign
DNA stably integrated into its genome.
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| Claim 8
A method for producing a transgenic dicotyledonous plant comprising stably
integrated non-Agrobacterium foreign DNA, the method comprising:
growing a seed of a dicotyledonous plant comprising
non-Agrobacterium foreign DNA stably integrated into its genome, said
plant derived from a dicotyledonous plant which was transformed by
Agrobacterium-mediated transformation with a gene vector comprising
(i) said non-Agrobacterium foreign DNA and
(ii) not
comprising a functional cytokinin autonomy gene.
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| Claim 9
A method for producing seed of a transgenic dicotyledonous plant comprising
stably integrated non-Agrobacterium foreign DNA, the method comprising:
A) propagating a dicotyledonous plant comprising non-Agrobacterium
foreign DNA stably integrated into its genome, said plant derived from a
dicotyledonous plant which was transformed by Agrobacterium-mediated
transformation with a gene vector comprising
(i) said
non-Agrobacterium foreign DNA and
(ii) not comprising a functional
cytokinin autonomy gene; and
B) harvesting seed from said propagated plant.
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| Claim 10
A method of transforming a dicotyledonous plant of a species that is a
naturally susceptible host for Agrobacterium, comprising:
contacting the plant with an Agrobacterium bacterium comprising a
gene vector, the vector comprising
(i) DNA foreign to the
Agrobacterium and
(ii) the vector not comprising a functional
cytokinin autonomy gene.
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| Claim 11
A method for producing a transgenic dicotyledonous plant comprising
non-Agrobacterium foreign DNA stably integrated in the plant's genome,
said method comprising the following steps:
A) transforming a cell of a dicotyledonous plant species that is a naturally
susceptible host for Agrobacterium by Agrobacterium -mediated
transformation with a gene vector comprising
(i) non-Agrobacterium
foreign DNA and
(ii) not comprising a functional cytokinin autonomy gene;
and
B) regenerating said transformed plant cell to produce a normal transformed
dicotyledonous plant with said foreign DNA stably integrated in the plant's
genome.
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| Claim 14
A method for producing a transgenic dicotyledonous plant comprising a stably
integrated non-Agrobacterium foreign DNA, the method comprising:
A) sexually propagating a dicotyledonous plant comprising
non-Agrobacterium foreign DNA stably integrated into its genome, said
plant derived from a dicotyledonous plant which is of a species that is a
naturally susceptible host for Agrobacterium and which was transformed
by Agrobacterium-mediated transformation with a gene vector comprising
(i) said non-Agrobacterium foreign DNA and
(ii) not
comprising a functional cytokinin autonomy gene; and
B) obtaining a progeny plant which comprises the non-Agrobacterium
foreign DNA stably integrated into its genome.
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| Claim 15
A method for producing a transgenic dicotyledonous plant comprising a stably
integrated non-Agrobacterium foreign DNA, the method comprising:
A) propagating a dicotyledonous plant comprising non-Agrobacterium
foreign DNA stably integrated into its genome, said plant derived from a
dicotyledonous plant which is of a species that is a naturally susceptible host
for Agrobacterium and which was transformed by
Agrobacterium-mediated transformation with a gene vector comprising
(i) said non-Agrobacterium foreign DNA and
(ii) not
comprising a functional cytokinin autonomy gene; and
B) obtaining a plant which comprises the non-Agrobacterium foreign
DNA stably integrated into its genome.
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| Claim 16
A method for producing a transgenic dicotyledonous plant comprising stably
integrated non-Agrobacterium foreign DNA, the method comprising:
growing a seed of a dicotyledonous plant comprising
non-Agrobacterium foreign DNA stably integrated into its genome, said
plant derived from a dicotyledonous plant which is of a species that is a
naturally susceptible host for Agrobacterium and which was transformed
by Agrobacterium-mediated transformation with a gene vector comprising
(i) said non-Agrobacterium foreign DNA and
(ii) not
comprising a functional cytokinin autonomy gene.
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| Claim 17
A method for producing seed of a transgenic dicotyledonous plant comprising
stably integrated non-Agrobacterium foreign DNA, the method comprising:
A) propagating a dicotyledonous plant comprising non-Agrobacterium
foreign DNA stably integrated into its genome, said plant derived from a
dicotyledonous plant which is of a species that is a naturally susceptible host
for Agrobacterium and which was transformed by
Agrobacterium-mediated transformation with a gene vector comprising
(i) said non-Agrobacterium foreign DNA and
(ii) not
comprising a functional cytokinin autonomy gene; and
B) harvesting seed from said propagated plant.
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| Claim 18
An Agrobacterium-mediated method for genetically engineering a
dicotyledonous plant comprising:
A) producing a transgenic plant cell by transforming a cell of dicotyledonous
plant species that is a naturally susceptible host for Agrobacterium
with a gene vector comprising non-Agrobacterium foreign DNA and not
comprising a functional cytokinin autonomy gene by Agrobacterium
tumefaciens-mediated transformation; and
B) regenerating a whole
normal plant from the transgenic plant cell which contains said foreign DNA
stably integrated into its genome.
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| Claim 20
A method for producing a transgenic dicotyledonous plant comprising intact
T-DNA comprising non-Agrobacterium foreign DNA stably integrated into
the genome of said plant, the method comprising:
A) propagating a dicotyledonous plant comprising non-Agrobacterium
foreign DNA stably integrated into its genome, said plant derived from a
dicotyledonous plant which is of a species that is a naturally susceptible host
for Agrobacterium and which was transformed by
Agrobacterium-mediated transformation with a disarmed T-DNA gene vector
comprising
(i) said non-Agrobacterium foreign DNA and
(ii)
not comprising a functional cytokinin autonomy gene; and
B) obtaining a plant which comprises the intact T-DNA stably integrated into
its genome.
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The United States patent
US
6051757 claims
- transformation of a dicot plant, which is either susceptible to
transformation (claim 1) or a naturally susceptible host (claim 10) with A.
tumefaciens (claim 1) or an Agrobacterium (claim 10) having a
vector that lacks the cytokinin function and contains foreign DNA instead;
- regeneration of a transformed dicot cell and production of a morphologically
normal plant;
- sexual or non-sexual propagation of the transformed dicot plant and
generation of a progeny bearing the foreign gene;
- seed production and harvesting from a propagated transformed dicot plant.
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Washington University, exclusively licensed to
Syngenta
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| Remarks |
A related United States application (US 07/155092) was in
interference, the process by which the United States Patent Office determines
who was the earliest inventor when there are competing claims (in this case,
from Monsanto).
According to the USPTO status database PAIR, Syngenta lost the interference case
in 2004.
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Note: Patent information on this page was last updated on 10 March 2006.
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