CA 2290354
- Earliest priority - 14 Apr 1998
- Filed - 12 Apr 1999
- Application pending
- Expected expiry - not applicable
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Title - Diagnostic systems and method for determining the
presence of a genotoxic and/or toxic compound in a sample
Claim 1
A diagnostic system made of:
- a transformed micro-organism capable of an increased reporter activity upon
exposure to an environmental insult, said micro-organism having a stress
inducible promoter sequence being operatively linked to a reporter encoding
nucleic acid sequence encoding a reporter molecule resulting in a signal that
can be assayed, and of
- a transformed micro-organism having a constitutive and non stress inducible
promoter sequence being operatively linked to a reporter encoding nucleic acid
sequence encoding a reporter molecule resulting in a signal that can be assayed.
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Claim 12
Method for determining the kinetics of genotoxicity of a compound into a
sample, wherein the measuring of luminescence of both inducible and constitutive
transformed micro-organisms occurs at multiple points in time, preferably
continuously, and in addition carrying out the step of determining the
signal-to-noise (S/N) ratio for the transformed micro-organisms at said point
and time, dividing the S/N ratio of the inducible micro-organism by teat of the
constitutive micro-organisms and plotting these data, said plotting representing
the corrected kinetics ef genotoxicity of the genotoxic compound in the sample.
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The claims are generally drawn towards:
- a diagnostic system made of two transformed microorganisms , one with a
stress inducible promoter and another with a constitutive and non-stress
inducible promoter operably linked to a reporter (claim 1)
- a method for determining the kinetics of genotoxicity of a compound into a
sample (claim 12)
Definitions extracted from the description are provided in US 6472152.
Comments:
Since this is a published application and not a granted patent, currently
there are no enforceable rights.
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VITO
BOERETANG 200
B-2400
MOL BELGIUM
Ph + 32 14 33 55 11
Fax + 32 14 33 55 99
vito@vito.be
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EP 950717
- Earliest priority - 14 Apr 1998
- Filed - 14 Apr 1998
- Application deemed to be withdawn - 25 Oct 2000
- Expected expiry - not applicable
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Title - Diagnostic systems and method for determining the
presence of a genotoxic and/or toxic compound in a sample
Claim 1
A diagnostic system made of :
a transformed micro-organism capable of an increased reporter activity upon
exposure to an environmental insult, said micro-organism having a stress
inducible promoter sequence being operatively linked to a reporter encoding
nucleic acid sequence encoding a reporter molecule resulting in a signal that
can be assayed, and of
a transformed micro-organism having a constitutive and non stress inducible
promoter sequence being operatively linked to a reporter encoding nucleic acid
sequence encoding a reporter molecule resulting in a signal that can be assayed.
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Claim 12
Method for determining the kinetics of genotoxicity of a compound into a
sample, wherein the measuring of luminescence of both inducible and constitutive
transformed micro-organisms occurs at multiple points in time, preferably
continuously, and in addition carrying out the step of determining the
signal-to-noise (S/N) ratio for the transformed micro-organisms at said point
and time, dividing the S/N ratio of the inducible micro-organism by that of the
constitutive micro-organisms and plotting these data, said plotting representing
the corrected kinetics of genotoxicity of the genotoxic compound in the sample.
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The claims are generally drawn towards:
- a diagnostic system made of two transformed microorganisms , one with a
stress inducible promoter and another with a constitutive and non-stress
inducible promoter operably linked to a reporter (claim 1)
- a method for determining the kinetics of genotoxicity of a compound into a
sample (claim 12)
Definitions extracted from the description are provided in US 6472152.
Comments:
Since this is a published application and not a granted patent, currently
there are no enforceable rights.
|
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US
6472152
- Earliest priority - 14 Apr 1998
- Filed - 12 Apr 1999
- Granted - 29 Oct 2002
- Expected expiry - 12 Apr 2019
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Title - Diagnostic systems and method for determining the
presence of a genotoxic and/or toxic compound in a sample
Claim 1
A diagnostic system comprising:
- a first transformed micro-organism capable of an increased reporter activity
upon exposure to an environmental insult, said micro-organism
having a stress inducible promoter sequence being operatively
linked to a nucleic acid sequence encoding a reporter molecule
resulting in a signal that can be assayed, and
- a second transformed micro-organism having a constitutive and non-stress
inducible promoter sequence being operatively linked to a nucleic acid sequence
encoding a reporter molecule resulting in a signal that can be assayed, wherein
said second and first microorganisms are Salmonella typhimurium
microorganisms selected from the group consisting of
- Ames test micro-organism, micro-organism designated by deposit number LMG
P-18318, transformed TA98, transformed TA100, transformed TA102, transformed
TA104, transformed TA1538, transformed TA7001 to transformed TA7005, and
transformed TA7041 to transformed TA7046,
said micro-organisms being suitable to cooperatively detect said
environmental insult while eliminating false positive and false negative result.
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The claims are generally drawn towards:
- a diagnostic system comprising two transformed S. typhimurium
strains, one with a stress inducible promoter and another with a constitutive
and non-stress inducible promoter operably linked to a nucleic acid encoding a
reporter molecule (claim 1)
Definitions extracted from the description are:
- Environmental insult - this term is not defined in the specification.
Dependent claim 7 recites this term as 'the presence of a genotoxic and/or a
toxic compound in a sample'
- Stress inducible promoter - this term is not defined in the specification.
Examples of promoters are provided in the following statement: 'Advantageously,
the stress inducible promoter sequence in the transformed bioluminescent
micro-organism of the diagnostic system according to the invention is selected
from the group consisting of groEL, dnaK, grpE, phoA, glnA, lon, lysU, rpoD,
clpB, clpP, uspA, katG, uvrA, frda, micF, fabA, lac, his, sodA, sodB, soi-28,
recA, xthA, narG, recF, recj, recN, recO, recQ, ruv, uvrD, ars, cad, mer,
pco, and sfiA.'
- Reporter - this term is not defined in the specification. Examples of
reporters are provided in the following statement: 'Preferably, both transformed
micro-organisms are bioluminescent micro-organisms and the signal can be assayed
as light production. Other possibilities are the peroxydase, alkaline
phosphatase, β-gal and gus genetic sequence...'
- LMG P-18318 - "pr1" strain, which is a transgenic S. typhimurium TA104
containing a constitutive promoter and luxCDABE
Comments:
Independent claim 1 of US 6472152 is relatively narrow in comparison to the
other patent applications presented in this section (note: US 6472152 is the
only granted patent presented in this section) in terms of the microorganism
that can be used, which is limited to particular strains of S.
typhimurium.
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WO
1999/53092
- Earliest priority - 14 Apr 1998
- Filed - 12 Apr 1999
- Published - 21 Oct 1999
- Expected expiry - not applicable
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Title - Diagnostic systems and method for determining the
presence of a genotoxic and/or toxic compound in a sample
Claim 1
A diagnostic system made of
- a transformed micro-organism capable of an increased reporter activity upon
exposure to an environmental insult, said micro-organism having a stress
inducible promoter sequence being operatively linked to a reporter encoding
nucleic acid sequence encoding a reporter molecule resulting in a signal that
can be assayed, and of
- a transformed micro-organism having a constitutive and non stress inducible
promoter sequence being operatively linked to a reporter encoding nucleic acid
sequence encoding a reporter molecule resulting in a signal that can be assayed.
|
Claim 12
Method for determining the kinetics of genotoxicity of a compound into a
sample, wherein the measuring of luminescence of both inducible and constitutive
transformed micro-organisms occurs at multiple points in time, preferably
continuously, and in addition carrying out the step of determining the
signal-to-noise (S/N) ratio for the transformed micro-organisms at said point
and time, dividing the S/N ratio of the inducible micro-organism by that of the
constitutive micro-organisms and plotting these data, said plotting representing
the corrected kinetics of genotoxicity of the genotoxic compound in the sample.
|
The claims are generally drawn towards:
- a diagnostic system made of two transformed microorganisms , one with a
stress inducible promoter and another with a constitutive and non-stress
inducible promoter operably linked to a reporter (claim 1)
- a method for determining the kinetics of genotoxicity of a compound into a
sample (claim 12)
Definitions extracted from the description are provided in US 6472152.
Comments:
Since this is a published application and not a granted patent, there are no
enforceable rights.
|
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