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CA 2287940
- Earliest priority - 1 May 1997
- Filed - 29 Apr 1998
- Application lapsed - 17 Mar 2004
- Expected expiry - not applicable
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Title - Bioluminescent reporter bacterium and methods for
toxicity monitoring in biological wastewater treatment systems
Claim 1
A reporter bacterium, comprising
- a bacterium that occurs naturally in a biological
sludge and that contains a nucleic acid that encodes a
reporter protein not found in the naturally occurring
bacterium.
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Claim 8
A bioluminescent reporter bacterium, obtained by the process comprising:
- contacting a bacteria-containing sample of a biological sludge with a donor
bacterium, wherein
the donor bacterium contains a nucleic acid that encodes a
bioluminescent reporter protein that does not naturally occur in biological
sludge bacteria under conditions whereby mating can occur.
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Claim 12
A bioluminescent reporter bacterium, obtained by the process comprising:
- delivering to an isolated bacterium, that occurs naturally in a biological
sludge, a nucleic acid construct which contains a nucleic acid that encodes a
bioluminescent reporter protein that does not occur naturally in the bacterium,
under conditions whereby the nucleic acid encoding the bioluminescent reporter
protein is taken up by a bacterium in the sludge sample.
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Claim 15
A method for making a bioluminescent reporter bacterium, comprising:
- contacting a bacteria-containing sample of a biological sludge with a
bacterium that contains a nucleic acid that encodes a bioluminescent reporter
protein that does not naturally occur in biological sludge bacteria, under
conditions whereby the nucleic acid encoding the reporter protein is taken up by
a bacterium in the sludge sample.
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Claim 16
A method for making a bioluminescent reporter bacterium, comprising:
- delivering to an isolated bacterium, that occurs naturally in a biological
sludge, a nucleic acid construct which contains a nucleic acid that encodes a
bioluminescent reporter protein that does not occur naturally in the bacterium,
under conditions whereby the nucleic acid encoding the reporter protein is taken
up by the isolated sludge bacterium.
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The claims are generally drawn towards:
- a reporter bacterium that occurs naturally in a biological sludge and
contains a nucleic acid that encodes a reporter protein not found in the
naturally occurring bacterium (claim 1)
- a bioluminescent reporter bacterium (claim 8, 12)
- a method for making a bioluminescent reporter bacterium (claim 15, 16)
Definitions extracted from the claims are:
- Biological sludge - includes sludges from wastewater treatment facilities
(e.g., industrial, municipal, etc.) and is also commonly referred to as
biosolids and wastewater solids.
- Bacterium - can be any bacteria which do not naturally contain a
bioluminescent reporter gene, and in a preferred embodiment are those bacteria
which occur naturally in a biological sludge.
- Donor bacterium - can be selected from among the bacteria used as donors in
other contexts, for example, Escherichia coli, Alcaligenes
eutrophus and Pseudomonas putida, although, theoretically, almost
all known bacteria could serve as donors.
- Reporter protein - can describe one protein that is sufficient to produce
the signal, or more than one protein, which when expressed sequentially or
simultaneously or with some temporal overlap, produces the signal.
Comments:
Since this is a published application and not a granted patent, currently
there are no enforceable rights.
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Eastman Chemical Co.
P.O. BOX 511
KINGSPORT, TENNESSEE 37662
Licensing information:
Eastman Chemical Company
Technology Licensing and Alliances
P.O. Box 431, Bldg 280
Kingsport, TN 37662
Ph 800-Eastman(800-327-8626), Ext. 6076
Fax +1-423-229-2811
Email licensing@eastman.com
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US
6110661
- Earliest priority - 1 May 1997
- Filed - 2 Oct 1997
- Granted - 29 Aug 2000
- Expected expiry - 2 Oct 2017
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Title - Bioluminescent reporter bacterium
Claim 1
A method of making and using bioluminescent reporter bacteria, the method
comprising the following steps:
a) obtaining bacteria from biological wastewater sludge of a wastewater
treatment facility;
b) introducing into said bacteria a nucleic acid construct that encodes a
bioluminescent reporter protein under conditions whereby the nucleic acid
encoding the bioluminescent reporter protein is taken up by, and expressed in,
said bacteria, and wherein the bioluminescent reporter protein does not
naturally occur in said bacteria;
c) contacting the influent from the wastewater treatment facility with the
bioluminescent reporter bacteria obtained by steps a) and b);
d) detecting the bioluminescent reporter protein expressed by the bioluminescent
reporter bacteria; and
e) correlating the presence of toxicity with the reduction in expression of the
bioluminescent reporter protein;
wherein the bioluminescent reporter bacteria are made from bacteria obtained
from the same wastewater treatment facility at which the bioluminescent reporter
bacteria are to be used for detecting toxicity, and are therefore specifically
adapted to the conditions of said wastewater treatment facility.
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The claims are generally drawn towards:
- a method of making and using bioluminescent reporter bacteria (claim 1)
Definitions extracted from the specification are provided in CA 2287940.
Comments:
Independent claim 1 (which is the only independent claim in granted US
6110661) is a method claim using the bioluminescent reporter bacterium, as
opposed to the PCT application and the subsequent national phase entries that
contain claims that are generally directed towards the bioluminescent reporter
bacterium (see below for PCT application WO 1998/49337, note that applications
do not have enforceable rights until they are granted). Therefore the scope of
granted US 6110661 is relatively narrow in comparison to the other patent
applications described in this section.
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WO
1998/49337
- Earliest priority - 1 May 1997
- Filed - 29 Apr 1998
- Published - 5 Nov 1998
- Expected expiry - not applicable
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Title - Bioluminescent reporter bacterium and methods for
toxicity monitoring in biological wastewater treatment systems
Claim 1
A reporter bacterium, comprising
- a bacterium that occurs naturally in a biological sludge and that contains a
nucleic acid that encodes a reporter protein not found in the naturally
occurring bacterium.
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Claim 8
A bioluminescent reporter bacterium, obtained by the process comprising:
- contacting a bacteria-containing sample of a biological sludge with a donor
bacterium, wherein
the donor bacterium contains a nucleic acid that encodes a bioluminescent
reporter protein that does not naturally occur in biological sludge bacteria
under conditions whereby mating can occur.
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Claim 12
A bioluminescent reporter bacterium, obtained by the process comprising:
- delivering to an isolated bacterium, that occurs naturally in a biological
sludge, a nucleic acid construct which contains a nucleic acid that encodes a
bioluminescent reporter protein that does not occur naturally in the bacterium,
under conditions whereby the nucleic acid encoding the bioluminescent reporter
protein is taken up by a bacterium in the sludge sample.
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Claim 15
A method for making a bioluminescent reporter bacterium, comprising:
- contacting a bacteria-containing sample of a biological sludge with a
bacterium that contains a nucleic acid that encodes a bioluminescent reporter
protein that does not naturally occur in biological sludge bacteria, under
conditions whereby the nucleic acid encoding the reporter protein is taken up by
a bacterium in the sludge sample.
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Claim 16
A method for making a bioluminescent reporter bacterium, comprising:
- delivering to an isolated bacterium, that occurs naturally in a biological
sludge, a nucleic acid construct which contains a nucleic acid that encodes a
bioluminescent reporter protein that does not occur naturally in the bacterium,
under conditions whereby the nucleic acid encoding the reporter protein is taken
up by the isolated sludge bacterium.
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The claims are generally drawn towards:
- a reporter bacterium that occurs naturally in a biological sludge and
contains a nucleic acid that encodes a reporter protein not found in the
naturally occurring bacterium (claim 1)
- a bioluminescent reporter bacterium (claim 8, 12)
- a method for making a bioluminescent reporter bacterium (claim 15, 16)
Definitions extracted from the claims are provided in CA 2287940.
Comments:
Since this is a published application and not a granted patent, there are no
enforceable rights.
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