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Promoters and UAS from opine synthase genes - Specific Patent Information

Patent Number

Title, Independent Claims and Summary of Claims

Assignee

US 5955646

  • Earliest priority - 19 November 1993
  • Filed - 31 July 1996
  • Granted - 21 September 1999
  • Expected expiry - 19 November 2013

Title - Chimeric regulatory regions and gene cassettes for expression of genes in plants

Claim 1

A cassette for inducible expression of a foreign gene comprising said foreign gene operably linked to a regulatory region comprising
    a promoter derived from a mannopine synthase gene of Agrobacterium tumefaciens,
    an upstream activating sequence derived from a mannopine synthase gene of Agrobacterium tumefaciens, and
    at least one upstream activating sequence derived from an octopine synthase gene of Agrobacterium tumefaciens.

Claim 2

A method for nematode inducible expression of a foreign gene in a plant, comprising:

    linking said foreign gene to a regulatory region comprising a promoter derived from a mannopine synthase gene of Agrobacterium tumefaciens comprising 138 bases upstream of the transcription initiation site, and an upstream activating sequence derived from a mannopine synthase gene of Agrobacterium tumefaciens;
    inserting said foreign gene and said regulatory region in said plant, wherein expression is induced by nematode attack on the plant.

Claim 3

A method for nemotode inducible expression of a foreign gene in a plant, comprising:  
    linking said foreign gene to a regulatory region comprising
        a promoter derived from a mannopine synthase gene of Agrobacterium tumefaciens,
        an upstream activating sequence derived from a mannopine synthase gene of Agrobacterium tumefaciens, and
        at least one upstream activating sequence derived from an octopine synthase gene of Agrobacterium tumefaciens;  
    inserting said foreign gene and said regulatory region in said plant, wherein expression is induced by nematode attack on the plant.

Claim 4

A chimeric regulatory region for expressing genes in plants comprising at least three upstream activating sequences derived from an Agrobacterium tumsfaciens octopine synthase gene operably linked to a promoter derived from an Agrobacterium tumefaciens mannopine synthase gene.

Claim 5

A cassette for expressing a foreign gene comprising the foreign gene operably linked to a chimeric regulatory region comprising at least three upstream activating sequences derived from Agrobacterium tumefaciens octopine synthase genes operably linked to a promoter derived from an Agrobacterium tumefaciens mannopine synthase gene.

Claim 6

A plasmid comprising a cassette comprising a foreign gene operably linked to a chimeric regulatory region comprising at least three upstream activating sequences derived from Agrobacterium tumefaciens octopine synthase genes operably linked to a promoter derived from an Agrobacterium tumefaciens mannopine synthase gene.

Claim 7

A method of expressing a foreign gene in a plant, comprising:
    linking said foreign gene to a chimeric regulatory region comprising at least three upstream activating sequences derived from an Agrobacterium tumefaciens octopine synthase gene operably linked to a promoter derived from an Agrobacterium tumefaciens mannopine synthase gene; and         inserting said foreign gene and said chimeric regulatory region into a plant, wherein said plant expresses said foreign gene.

Claim 8

A transgenic plant comprising a cassette comprising a foreign gene operably linked to a chimeric regulatory region comprising at least three upstream activating sequences derived from Agrobacterium tumefaciens octopine synthase genes operably linked to a promoter derived from an Agrobacterium tumefaciens mannopine synthase gene.

Claim 9

A chimeric regulatory region for expressing genes in plants comprising at least three upstream activating sequences derived from an Agrobacterium tumefaciens octopine synthase gene operably linked to an upstream activating sequence derived from an Agrobacterium tumefaciens mannopine synthase gene that is operably linked to a promoter derived from an Agrobacterium tumefaciens mannopine synthase gene.

Claim 10

A cassette for expressing a foreign gene comprising the foreign gene operably linked to a chimeric regulatory region comprising at least three upstream activating sequences derived from Agrobacterium tumefaciens octopine synthase genes operably linked to an upstream activating sequence derived from an Agrobacterium tumefaciens mannopine synthase gene that is operably linked to a promoter derived from an Agrobacterium tumefaciens mannopine synthase gene.

Claim 11

A method of expressing a foreign gene in a plant, comprising:
    linking said foreign gene to a chimeric regulatory region comprising at least three upstream activating sequences derived from an Agrobacterium tumefaciens octopine synthase gene operably linked to an upstream activating sequence derived from an Agrobacterium tumefaciens mannopine synthase gene that is operably linked to a promoter derived from an Agrobacterium tumefaciens mannopine synthase gene; and
    inserting said foreign gene and said chimeric regulatory region into a plant, wherein said plant expresses said foreign gene.

Claim 12

A transgenic plant comprising a chimeric regulatory region for expressing genes in plants comprising at least three upstream activating sequences derived from an Agrobacterium tumefaciens octopine synthase gene operably linked to an upstream activating sequence derived from an Agrobacterium tumefaciens mannopine synthase gene that is operably linked to a promoter derived from an Agrobacterium tumefaciens mannopine synthase gene.

The claims are directed to:

  • A cassette for inducible expression of a foreign gene operably linked to a regulatory region having
    • a promoter and an UAS  from the mas gene and
    • at least one UAS  from an ocs gene.

        The term "operably linked" is not explicitly defined but in the context of the disclosure appears to refer to a gene sequence present on the same nucleic acid molecule as a promoter sequence and whose expression is under the control of that promoter sequence.

  • A chimeric regulatory region for expressing genes in plants having at least 3 UAS derived from an ocs gene linked to a promoter derived from a mas gene. A cassette, a plasmid,  a method of expressing a foreign gene in a plant using this regulatory region, methods for nematode inducible expression of a foreign gene, and a transgenic plant are also claimed.
  • A chimeric regulatory region for expressing genes in plants having
    • 4 UASs,
    • at least 3 UASs from an ocs gene and one UAS from a mas gene linked to a mas gene promoter.
    •  A cassette, a method of expressing a foreign gene in a plant using this regulatory region and a transgenic plant are also claimed.

        The disclosure describes that a "foreign gene"  includes "any DNA that is sought to be expressed in [a] transgenic plant. In this context, the gene, no matter the source, is inserted into the plant genome and is thus foreign to that plant in the location of insertion, even if the gene originated from the plant being transformed." So a foreign gene can be a plant gene and may even be from the plant being transformed if inserted into a different genomic location from where it is usually found. 

      A cassette, as described in the patent, does not necessarily include an origin of replication.  As claimed, a cassette could encompass a plasmid or a portion of a plasmid (e.g., a linear fragment).

Biotechnology Research and Development Corporation

& Purdue Research Foundation

AU 687961 B

  • Earliest priority - 19 November 1993
  • Filed - 17  November 1994
  • Granted - 5 March 1998
  • Expected expiry - 16 November 2014

Title - Chimeric regulatory regions and gene cassettes for expression of genes in plants

Claim 1

A chimeric regulatory region for expressing genes in plants comprising an upstream activating sequences derived from an Agrobacterium tumefaciens octopine synthase gene operably linked to a promoter derived from an Agrobacterium tumefaciens mannopine synthase gene.

Claim 2

A cassette for expressing a gene comprising a gene operably linked to a chimeric regulatory region comprising an upstream activating sequences derived from an Agrobacterium tumefaciens octopine synthase gene operably linked to a promoter derived from an Agrobacterium tumefaciens mannopine synthase gene.

Claim 4

A chimeric regulatory region for expressing genes in plants comprising at least two upstream activating sequences derived from an Agrobacterium tumefaciens opine synthase gene operably linked to a promoter derived from an Agrobacterium tumefaciens opine synthase gene, wherein at least one of said upstream activating elements are derived from a different opine synthase gene than said promoter.

Claim 8

A cassette for expressing a gene comprising a gene operably linked to a chimeric regulatory region comprising at least two upstream activating sequences derived from an Agrobacterium tumefaciens opine synthase gene operably linked to a promoter derived from an Agrobacterium tumefaciens opine synthase gene, wherein at least one of said upstream activating elements are derived from a different opine synthase gene than said promoter.

Claim 13

A cassette for inducible expression of a foreign gene comprising said foreign gene operably linked to a regulatory region comprising:

    a) a promoter derived from a mannopine synthase gene of Agrobacterium tumefaciens by deletion to nucleotide position -138, and
    b) an upstream activating sequence derived from a mannopine synthase gene of Agrobacterium tumefaciens.

Claim 15

A cassette for inducible expression of a foreign gene comprising said foreign gene operably linked to a regulatory region comprising:

    a) a promoter derived from a mannopine synthase gene of Agrobacterium tumefaciens,
    b) an upstream activating sequence derived from a mannopine synthase gene of Agrobacterium tumefaciens, and
    c) an upstream activating sequence derived from an octopine synthase gene of Agrobacterium tumefaciens.

Claim 16

A method for expressing a gene in a plant, comprising the steps of:

    a) linking said gene to a chimeric regulatory region comprising an upstream activating sequence derived from an Agrobacterium tumefaciens octopine synthase gene operably linked to a promoter derived from an Agrobacterium tumefaciens mannopine synthase gene;
    b) inserting said gene and said chimeric regulatory region into a plant; and
    c) allowing said plant to express said gene.

Claim 17
A method for expressing a gene in a plant, comprising the steps of:

    a) linking said gene to a chimeric regulatory region comprising at least two upstream activating sequences derived from an Agrobacterium tumefaciens opine synthase gene operably linked to a promoter derived from an Agrobacterium tumefaciens opine synthase gene, wherein at least one of said upstream activating elements are derived from a different opine synthase gene than said promoter;
    b) inserting said gene and said chimeric regulatory region into a plant; and
    c) allowing said plant to express said gene.

Claim 21

A method of inducible expression of a foreign gene in a plant, comprising:

    a) linking said foreign gene to a regulatory region comprising:
         i. a promoter derived from a mannopine synthase gene of Agrobacterium tumefaciens by deletion to nucleotide position -138, and
        ii. an upstream activating sequence derived from a mannopine synthase gene of Agrobacterium tumefaciens;  
     b) inserting said foreign gene and said regulatory region in said plant; and
    c) inducing expression of said foreign gene.

Claim 23

A method for inducible expression of a foreign gene in a plant, comprising:

    a) linking said foreign gene to a regulatory region comprising:
    i. a promoter derived from a mannopine synthase gene of Agrobacterium tumefaciens,
    ii. an upstream activating sequence derived from a mannopine synthase gene of Agrobacterium tumefaciens; and  
    iii. an upstream activating sequence derived from an octopine synthase gene of Agrobacterium tumefaciens;  
    b) inserting said foreign gene and said regulatory region in said plant; and
    c) inducing expression of said foreign gene.

Claim 32

A chimeric regulatory region for expressing genes in plants comprising at least three upstream activating sequences derived from an Agrobacterium tumefaciens octopine synthase gene operably linked to a promoter derived from an Agrobacterium tumefaciens mannopine synthase gene.

Claim 33

A cassette for expressing a gene comprising a gene operably linked to a chimeric regulatory region comprising at least three upstream activating sequences derived from Agrobacterium tumefaciens octopine synthase genes operably linked to a promoter derived from an Agrobacterium tumefacie ns mannopine synthase gene.

Claim 34

A method of expressing a gene in a plant, comprising the steps of:

    a) linking said gene to a chimeric regulatory region comprising at least three upstream activating sequences derived from an Agrobacterium tumefaciens octopine synthase gene operably linked to  a promoter derived from an Agrobacterium tumefaciens mannopine synthase;
    b) inserting said gene and said chimeric regulatory region into a plant; and
    c) allowing said plant to express said gene.

Claim 37

A chimeric regulatory region for expressing a gene in a plant comprising at least three upstream activating sequences derived from an Agrobacterium tumefaciens opine synthase gene operably linked to a promoter derived from an Agrobacterium tumefaciens opine synthase gene, wherein at least one of said upstream activating elements are derived from a different opine synthase gene than said promoter.

Claim 39

A cassette for expressing a gene in a plant comprising a gene operably linked to a chimeric regulatory region comprising at least three upstream activating sequences derived from Agrobacterium tumefaciens opine synthase genes operably linked to a promoter derived from an Agrobacterium tumefaciens opine synthase gene, wherein at least one of said upstream activating elements are derived from a different opine synthase gene than said promoter.

Claim 40

A chimeric regulatory region for expressing a gene in a plant comprising at least three upstream activating sequences derived from an Agrobacterium tumefaciens opine synthase gene operably linked to a promoter derived from an Agrobacterium tumefaciens opine synthase gene.

Claim 41

A chimeric regulatory region for expressing a gene in a plant comprising at least two upstream activating sequences derived from an Agrobacterium tumefaciens opine synthase gene operably linked to a promoter derived from an Agrobacterium tumefaciens opine synthase gene.

The claim elements are:

  • A chimeric regulatory region for expressing genes in plants comprising a promoter derived from a mas gene and an UAS derived from an ocs gene.
  • A chimeric regulatory region for expressing genes in plants having at least 2 UAS elements and a promoter from an opine synthase gene. One of the UAS elements derives from a different opine gene than the promoter.
  • A cassette for inducible expression of a foreign gene linked to a regulatory region having a promoter and a UAS from a mas gene.
  • A cassette for inducible expression of a foreign gene linked to a regulatory region having a promoter and a UAS from a mas gene and a UAS from an ocs gene.
  • A chimeric regulatory region for expressing genes in plants having at least 3 UAS elements from an ocs gene linked to a promoter from a mas gene.
  • A chimeric regulatory region for expressing genes in plants having at least 3 UAS elements and a promoter from an opine synthase gene. One of the UAS is from a different opine gene than the promoter.
  • Chimeric regulatory regions comprising at least 2 and at least 3 UAS elements from an opine synthase gene and a promoter also from an opine synthase gene.
  • Cassettes containing the mentioned chimeric regulatory regions and methods for expressing a gene in a plant are also claimed.

EP 729514 B1

  • Earliest priority - 19 November 1993
  • Filed - 4 September 1996
  • Granted - 12 August 2005
  • Expected expiry - 3 September 2016

Title - Chimeric regulatory regions and gene cassettes for expression of genes in plants

The number of claims in this granted European patent was reduced to nine with only one independent claim as follows:

Claim 1

A chimeric regulatory region for expressing genes in plants comprising an upstream activating sequence of an Agrobacterium tumefaciens opine synthase gene operably linked to a promoter derived from a second Agrobacterium tumefaciens opine synthase gene operably linked to a promoter of an Agrobacterium tumefaciens mannopine synthase.

CA 2174954

  • Earliest priority - 19 November 1993
  • Filed - 24 April 1996
  • Granted - 15 March 2005
  • Expected expiry - 23 April 2016

Title - Chimeric regulatory regions and gene cassettes for expression of genes in plants

Claim 1

A chimeric regulatory region for expressing genes in plants comprising an upstream activating sequence derived from an Agrobacterium tumefaciens octopine synthase gene operably linked to a promoter derived from an Agrobacterium tumefaciens mannopine synthase gene.

Claim 2

A chimeric regulatory region for expressing genes in plants comprising at least two upstream activating sequences derived from an Agrobacterium tumefaciens opine synthase gene operably linked to a promoter derived from an Agrobacterium tumefaciens opine synthase gene, wherein at least one of said upstream activating elements are derived from a different opine synthase gene than said promoter.

Claim 3

A chimeric regulatory region for expressing genes in plants comprising at least three upstream activating sequences derived from an Agrobacterium tumefaciens octopine synthase gene operably linked to a promoter derived from an Agrobacterium tumefaciens mannopine synthase gene.

Claim 7

A cassette for expressing a gene in plants, said cassette comprising a gene operably linked to chimeric regulatory region comprising an upstream activating sequence derived from an Agrobacterium tumefaciens octopine synthase gene operably linked to a promoter derived from an Agrobacterium tumefaciens mannopine synthase gene.

Claim 8

A cassette for expressing a gene in plants, said cassette comprising a gene operably linked to chimeric regulatory region comprising at least two upstream activating sequences derived from an Agrobacterium tumefaciens opine synthase gene operably linked to a promoter derived from an Agrobacterium tumefaciens opine synthase gene, wherein at least one of said upstream activating elements are derived from a different opine synthase gene than said promoter.

Claim 12

A cassette for inducible expression of a foreign gene in plants, said cassette comprising said foreign gene operably linked to a regulatory region comprising
    a promoter derived from a mannopine synthase gene of Agrobacterium tumefaciens by deletion to base pair position -138 and
    an upstream activating sequence derived from a mannopine synthase gene of Agrobacterium tumefaciens.

Claim 13

A cassette for inducible expression of a foreign gene in plants, said cassette comprising said foreign gene operably linked to a regulatory region comprising
    a promoter derived from a mannopine synthase gene of Agrobacterium tumefaciens,
    an upstream activating sequence derived from a mannopine synthase gene of Agrobacterium tumefaciens, and
    an upstream activating sequence derived from an octopine synthase gene of Agrobacterium tumefaciens.

Claim 14

A cassette for expressing a gene in plants, said cassette comprising a gene operably linked to a chimeric regulatory region comprising at least three upstream activating sequences derived from Agrobacterium tumefaciens octopine synthase genes operably linked to a promoter derived from an Agrobacterium tumefaciens mannopine synthase gene.

Claim 16

A method for expressing a gene in a plant, comprising the steps of:

    linking said gene to a chimeric regulatory region comprising an upstream activating sequence derived from an Agrobacterium tumefaciens octopine synthase gene operably linked to a promoter derived from an Agrobacterium tumefaciens mannopine synthase gene;
    inserting said gene and said chimeric regulatory region into a plant; and
    allowing said plant to express said gene.

Claim 17

A method for expressing a gene in a plant, comprising the steps of:

    linking said gene to a chimeric regulatory region comprising at least two upstream activating sequences derived from an Agrobacterium tumefaciens opine synthase gene operably linked to a promoter derived from an Agrobacterium tumefaciens opine synthase gene, wherein at least one of said upstream activating elements are derived from a different opine synthase gene than said promoter;
    inserting said gene and said chimeric regulatory region into a plant; and
    allowing said plant to express said gene.

Claim 21

A method for inducible expression of a foreign gene in a plant, comprising:

    linking said foreign gene to a regulatory region comprising a promoter derived from a mannopine synthase gene of Agrobacterium tumefaciens by deletion to base pair position -138 and an upstream activating sequence derived from a mannopine synthase gene of Agrobacterium tumefaciens;
    inserting said foreign gene and said regulatory region in said plant; and
    inducing expression of said foreign gene.

Claim 22

A method for inducible expression of a foreign gene in a plant, comprising:

    linking said foreign gene to a regulatory region comprising
        a promoter derived from a mannopine synthase gene of Agrobacterium tumefaciens,
        an upstream activating sequence derived from a mannopine synthase gene of Agrobacterium tumefaciens, and
        an upstream activating sequence derived from an octopine synthase gene of Agrobacterium tumefaciens;
    inserting said foreign gene and said regulatory region in said plant; and
    inducing expression of said foreign gene.

Claim 24

A method of expressing a gene in a plant, comprising the steps of:

    linking said gene to a chimeric regulatory region comprising at least three upstream activating sequences derived from an Agrobacterium tumefaciens octopine synthase gene operably linked to a promoter derived from an Agrobacterium tumefaciens mannopine synthase;
    inserting said gene and said chimeric regulatory region into a plant; and
    allowing said plant to express said gene.

This Canadian patent claims an additional method of expressing a gene in a plant using a chimeric regulatory region with at least 3 UAS from ocs gene and a promoter from a mas gene.

Remarks

Related patents were also granted in Russia (RU 2142998 C1), in China (CN 1061376) and in Japan (JP 9505205). An application was filed in Brazil but was abandoned on March 6, 2001.

Note: Patent information was last updated on 22 May 2006. Search terms: "gene" in abstract and "Purdue" or "Biotechnology research" in applicant. Patent database: PatentLens in combination with INPADOC.

The information contained in this page was believed to be correct at the time it was collated. New patents and patent applications, altered status of patents, and case law may have resulted in changes in the landscape. CAMBIA makes no warranty that it is correct or up to date at this time and accepts no liability for any use that might be made of it. Corrections or updates to the information are welcome. Please send an email to info@bios.net.

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