|
US
5955646
- Earliest priority - 19 November 1993
- Filed - 31 July 1996
- Granted - 21 September 1999
- Expected expiry - 19 November 2013
|
Title - Chimeric regulatory regions and gene cassettes for
expression of genes in plants
| Claim 1
A cassette for inducible expression of a foreign gene
comprising said foreign gene operably linked to a regulatory
region comprising
a promoter derived from a mannopine
synthase gene of Agrobacterium tumefaciens,
an upstream
activating sequence derived from a mannopine synthase gene of Agrobacterium
tumefaciens, and
at least one upstream activating sequence
derived from an octopine synthase gene of Agrobacterium tumefaciens.
|
| Claim 2
A method for nematode inducible expression of a foreign gene in a plant,
comprising:
linking said foreign gene to a regulatory region
comprising a promoter derived from a mannopine synthase gene of
Agrobacterium tumefaciens comprising 138 bases
upstream of the transcription initiation site, and an upstream activating
sequence derived from a mannopine synthase gene of Agrobacterium
tumefaciens;
inserting said foreign gene and said regulatory
region in said plant, wherein expression is induced by nematode attack on the
plant.
|
Claim 3
A method for nemotode inducible expression of a foreign gene in a plant,
comprising:
linking said foreign gene to a
regulatory region comprising
a promoter derived
from a mannopine synthase gene of Agrobacterium tumefaciens,
an upstream activating sequence derived from a mannopine synthase gene of
Agrobacterium tumefaciens, and
at least one upstream
activating sequence derived from an octopine synthase gene of Agrobacterium
tumefaciens;
inserting said foreign gene and said regulatory
region in said plant, wherein expression is induced by nematode attack on the
plant.
|
Claim 4
A chimeric regulatory region for expressing genes in plants
comprising at least three upstream activating sequences derived
from an Agrobacterium tumsfaciens octopine synthase gene operably
linked to a promoter derived from an Agrobacterium tumefaciens
mannopine synthase gene.
|
Claim 5
A cassette for expressing a foreign gene comprising the
foreign gene operably linked to a chimeric regulatory region
comprising at least three upstream activating sequences derived
from Agrobacterium tumefaciens octopine synthase genes operably linked
to a promoter derived from an Agrobacterium tumefaciens mannopine
synthase gene.
|
Claim 6
A plasmid comprising a cassette comprising
a foreign gene operably linked to a chimeric regulatory region
comprising at least three upstream activating sequences derived
from Agrobacterium tumefaciens octopine synthase genes operably linked
to a promoter derived from an Agrobacterium tumefaciens mannopine
synthase gene.
|
Claim 7
A method of expressing a foreign gene in a plant,
comprising:
linking said foreign gene to a chimeric
regulatory region comprising at least three upstream activating
sequences derived from an Agrobacterium tumefaciens octopine synthase
gene operably linked to a promoter derived from an Agrobacterium
tumefaciens mannopine synthase gene; and inserting said foreign
gene and said chimeric regulatory region into a plant, wherein said plant
expresses said foreign gene.
|
Claim 8
A transgenic plant comprising a cassette
comprising a foreign gene operably linked to a chimeric
regulatory region comprising at least three upstream activating
sequences derived from Agrobacterium tumefaciens octopine synthase
genes operably linked to a promoter derived from an Agrobacterium
tumefaciens mannopine synthase gene.
|
Claim 9
A chimeric regulatory region for expressing genes in plants
comprising at least three upstream activating sequences derived
from an Agrobacterium tumefaciens octopine synthase gene operably
linked to an upstream activating sequence derived from an Agrobacterium
tumefaciens mannopine synthase gene that is operably linked to a promoter
derived from an Agrobacterium tumefaciens mannopine synthase gene.
|
Claim 10
A cassette for expressing a foreign gene comprising the
foreign gene operably linked to a chimeric regulatory region
comprising at least three upstream activating sequences derived
from Agrobacterium tumefaciens octopine synthase genes operably linked
to an upstream activating sequence derived from an Agrobacterium
tumefaciens mannopine synthase gene that is operably linked to a promoter
derived from an Agrobacterium tumefaciens mannopine synthase gene.
|
Claim 11
A method of expressing a foreign gene in a plant,
comprising:
linking said foreign gene to a chimeric
regulatory region comprising at least three upstream activating
sequences derived from an Agrobacterium tumefaciens octopine synthase
gene operably linked to an upstream activating sequence derived from an
Agrobacterium tumefaciens mannopine synthase gene that is operably
linked to a promoter derived from an Agrobacterium tumefaciens
mannopine synthase gene; and
inserting said foreign gene and said
chimeric regulatory region into a plant, wherein said plant expresses said
foreign gene.
|
Claim 12
A transgenic plant comprising a chimeric regulatory region
for expressing genes in plants comprising at least three
upstream activating sequences derived from an Agrobacterium tumefaciens
octopine synthase gene operably linked to an upstream activating sequence
derived from an Agrobacterium tumefaciens mannopine synthase gene that
is operably linked to a promoter derived from an Agrobacterium
tumefaciens mannopine synthase gene.
|
The claims are directed to:
- A cassette for inducible expression of a foreign gene operably linked to a
regulatory region having
- a promoter and an UAS from the mas gene and
- at least one UAS from an ocs gene.
The term "operably linked" is not explicitly defined but in the
context of the disclosure appears to refer to a gene sequence present on the
same nucleic acid molecule as a promoter sequence and whose expression is under
the control of that promoter sequence.
- A chimeric regulatory region for expressing genes in plants having at least
3 UAS derived from an ocs gene linked to a promoter derived from a
mas gene. A cassette, a plasmid, a method of expressing a foreign gene
in a plant using this regulatory region, methods for nematode inducible
expression of a foreign gene, and a transgenic plant are also claimed.
- A chimeric regulatory region for expressing genes in plants having
- 4 UASs,
- at least 3 UASs from an ocs gene and one UAS from a mas gene linked
to a mas gene promoter.
- A cassette, a method of expressing a foreign gene in a plant using this
regulatory region and a transgenic plant are also claimed.
The disclosure describes that a "foreign gene" includes "any DNA
that is sought to be expressed in [a] transgenic plant. In this context, the
gene, no matter the source, is inserted into the plant genome and is thus
foreign to that plant in the location of insertion, even if the gene originated
from the plant being transformed." So a foreign gene can be a plant gene and may
even be from the plant being transformed if inserted into a different genomic
location from where it is usually found.
A cassette, as described in the patent, does not necessarily include an
origin of replication. As claimed, a cassette could encompass a plasmid or a
portion of a plasmid (e.g., a linear fragment).
|
Biotechnology
Research and Development Corporation
& Purdue
Research Foundation
|
|
AU 687961
B
- Earliest priority - 19 November 1993
- Filed - 17 November 1994
- Granted - 5 March 1998
- Expected expiry - 16 November 2014
|
Title - Chimeric regulatory regions and gene cassettes for
expression of genes in plants
| Claim 1
A chimeric regulatory region for expressing genes in plants
comprising an upstream activating sequences derived from an
Agrobacterium tumefaciens octopine synthase gene operably linked to a
promoter derived from an Agrobacterium tumefaciens mannopine synthase
gene.
|
| Claim 2
A cassette for expressing a gene comprising a gene operably
linked to a chimeric regulatory region comprising an upstream
activating sequences derived from an Agrobacterium tumefaciens
octopine synthase gene operably linked to a promoter derived from an
Agrobacterium tumefaciens mannopine synthase gene.
|
| Claim 4
A chimeric regulatory region for expressing genes in plants
comprising at least two upstream activating sequences derived
from an Agrobacterium tumefaciens opine synthase gene operably linked
to a promoter derived from an Agrobacterium tumefaciens opine synthase
gene, wherein at least one of said upstream activating elements are derived from
a different opine synthase gene than said promoter.
|
| Claim 8
A cassette for expressing a gene comprising a gene operably
linked to a chimeric regulatory region comprising at least two
upstream activating sequences derived from an Agrobacterium tumefaciens
opine synthase gene operably linked to a promoter derived from an
Agrobacterium tumefaciens opine synthase gene, wherein at least one of
said upstream activating elements are derived from a different opine synthase
gene than said promoter.
|
| Claim 13
A cassette for inducible expression of a foreign gene
comprising said foreign gene operably linked to a regulatory
region comprising:
a) a promoter derived from a mannopine synthase gene of
Agrobacterium tumefaciens by deletion to nucleotide position -138, and
b) an upstream activating sequence derived from a mannopine synthase
gene of Agrobacterium tumefaciens.
|
| Claim 15
A cassette for inducible expression of a foreign gene
comprising said foreign gene operably linked to a regulatory
region comprising:
a) a promoter derived from a mannopine synthase gene of
Agrobacterium tumefaciens,
b) an upstream activating sequence
derived from a mannopine synthase gene of Agrobacterium tumefaciens,
and
c) an upstream activating sequence derived from an octopine
synthase gene of Agrobacterium tumefaciens.
|
| Claim 16
A method for expressing a gene in a plant, comprising the
steps of:
a) linking said gene to a chimeric regulatory region comprising an
upstream activating sequence derived from an Agrobacterium tumefaciens
octopine synthase gene operably linked to a promoter derived from an
Agrobacterium tumefaciens mannopine synthase gene;
b)
inserting said gene and said chimeric regulatory region into a plant; and
c) allowing said plant to express said gene.
|
|
Claim 17
A method for expressing a gene in a plant,
comprising the steps of:
a) linking said gene to a chimeric regulatory region comprising at least
two upstream activating sequences derived from an Agrobacterium tumefaciens
opine synthase gene operably linked to a promoter derived from an
Agrobacterium tumefaciens opine synthase gene, wherein at least one of
said upstream activating elements are derived from a different opine synthase
gene than said promoter;
b) inserting said gene and said chimeric
regulatory region into a plant; and
c) allowing said plant to express
said gene.
|
| Claim 21
A method of inducible expression of a foreign gene in a plant,
comprising:
a) linking said foreign gene to a regulatory region
comprising:
i. a promoter derived from a
mannopine synthase gene of Agrobacterium tumefaciens by deletion to
nucleotide position -138, and
ii. an upstream activating sequence
derived from a mannopine synthase gene of Agrobacterium tumefaciens;
b) inserting said foreign gene and said regulatory region in said
plant; and
c) inducing expression of said foreign gene.
|
| Claim 23
A method for inducible expression of a foreign gene in a plant,
comprising:
a) linking said foreign gene to a regulatory region
comprising:
i. a promoter derived from a mannopine
synthase gene of Agrobacterium tumefaciens,
ii. an upstream
activating sequence derived from a mannopine synthase gene of Agrobacterium
tumefaciens; and
iii. an upstream activating sequence derived
from an octopine synthase gene of Agrobacterium tumefaciens;
b) inserting said foreign gene and said regulatory region in said plant; and
c) inducing expression of said foreign gene.
|
| Claim 32
A chimeric regulatory region for expressing genes in plants
comprising at least three upstream activating sequences derived
from an Agrobacterium tumefaciens octopine synthase gene operably
linked to a promoter derived from an Agrobacterium tumefaciens
mannopine synthase gene.
|
| Claim 33
A cassette for expressing a gene comprising a gene operably
linked to a chimeric regulatory region comprising at least
three upstream activating sequences derived from Agrobacterium
tumefaciens octopine synthase genes operably linked to a promoter derived
from an Agrobacterium tumefacie ns mannopine synthase gene.
|
| Claim 34
A method of expressing a gene in a plant, comprising the
steps of:
a) linking said gene to a chimeric regulatory region comprising at least
three upstream activating sequences derived from an Agrobacterium
tumefaciens octopine synthase gene operably linked to a promoter derived
from an Agrobacterium tumefaciens mannopine synthase;
b)
inserting said gene and said chimeric regulatory region into a plant; and
c) allowing said plant to express said gene.
|
| Claim 37
A chimeric regulatory region for expressing a gene in a plant
comprising at least three upstream activating sequences derived
from an Agrobacterium tumefaciens opine synthase gene operably linked
to a promoter derived from an Agrobacterium tumefaciens opine synthase
gene, wherein at least one of said upstream activating elements are derived from
a different opine synthase gene than said promoter.
|
| Claim 39
A cassette for expressing a gene in a plant comprising a
gene operably linked to a chimeric regulatory region comprising
at least three upstream activating sequences derived from Agrobacterium
tumefaciens opine synthase genes operably linked to a promoter derived from
an Agrobacterium tumefaciens opine synthase gene, wherein at least one
of said upstream activating elements are derived from a different opine synthase
gene than said promoter.
|
| Claim 40
A chimeric regulatory region for expressing a gene in a plant
comprising at least three upstream activating sequences derived
from an Agrobacterium tumefaciens opine synthase gene operably linked
to a promoter derived from an Agrobacterium tumefaciens opine synthase
gene.
|
| Claim 41
A chimeric regulatory region for expressing a gene in a plant
comprising at least two upstream activating sequences derived
from an Agrobacterium tumefaciens opine synthase gene operably linked
to a promoter derived from an Agrobacterium tumefaciens opine synthase
gene.
|
The claim elements are:
- A chimeric regulatory region for expressing genes in plants comprising a
promoter derived from a mas gene and an UAS derived from an
ocs gene.
- A chimeric regulatory region for expressing genes in plants having at least
2 UAS elements and a promoter from an opine synthase gene. One of the UAS
elements derives from a different opine gene than the promoter.
- A cassette for inducible expression of a foreign gene linked to a regulatory
region having a promoter and a UAS from a mas gene.
- A cassette for inducible expression of a foreign gene linked to a regulatory
region having a promoter and a UAS from a mas gene and a UAS from an
ocs gene.
- A chimeric regulatory region for expressing genes in plants having at least
3 UAS elements from an ocs gene linked to a promoter from a
mas gene.
- A chimeric regulatory region for expressing genes in plants having at least
3 UAS elements and a promoter from an opine synthase gene. One of the UAS is
from a different opine gene than the promoter.
- Chimeric regulatory regions comprising at least 2 and at least 3 UAS
elements from an opine synthase gene and a promoter also from an opine synthase
gene.
- Cassettes containing the mentioned chimeric regulatory regions and methods
for expressing a gene in a plant are also claimed.
|
|
EP
729514 B1
- Earliest priority - 19 November 1993
- Filed - 4 September 1996
- Granted - 12 August 2005
- Expected expiry - 3 September 2016
|
Title - Chimeric regulatory regions and gene cassettes for
expression of genes in plants
The number of claims in this granted European patent was reduced to nine with
only one independent claim as follows:
| Claim 1
A chimeric regulatory region for expressing genes in plants
comprising an upstream activating sequence of an Agrobacterium
tumefaciens opine synthase gene operably linked to a promoter derived from a
second Agrobacterium tumefaciens opine synthase gene operably linked to a
promoter of an Agrobacterium tumefaciens mannopine synthase.
|
|
|
|
CA
2174954
- Earliest priority - 19 November 1993
- Filed - 24 April 1996
- Granted - 15 March 2005
- Expected expiry - 23 April 2016
|
Title - Chimeric regulatory regions and gene cassettes for
expression of genes in plants
| Claim 1
A chimeric regulatory region for expressing genes in plants
comprising an upstream activating sequence derived from an
Agrobacterium tumefaciens octopine synthase gene operably linked to a
promoter derived from an Agrobacterium tumefaciens mannopine synthase
gene.
|
Claim 2
A chimeric regulatory region for expressing genes in plants
comprising at least two upstream activating sequences derived
from an Agrobacterium tumefaciens opine synthase gene operably linked
to a promoter derived from an Agrobacterium tumefaciens opine synthase
gene, wherein at least one of said upstream activating elements are derived from
a different opine synthase gene than said promoter.
|
Claim 3
A chimeric regulatory region for expressing genes in plants
comprising at least three upstream activating sequences derived
from an Agrobacterium tumefaciens octopine synthase gene operably
linked to a promoter derived from an Agrobacterium tumefaciens
mannopine synthase gene.
|
Claim 7
A cassette for expressing a gene in plants, said cassette
comprising a gene operably linked to chimeric regulatory region
comprising an upstream activating sequence derived from an
Agrobacterium tumefaciens octopine synthase gene operably linked to a
promoter derived from an Agrobacterium tumefaciens mannopine synthase
gene.
|
Claim 8
A cassette for expressing a gene in plants, said cassette
comprising a gene operably linked to chimeric regulatory region
comprising at least two upstream activating sequences derived
from an Agrobacterium tumefaciens opine synthase gene operably linked
to a promoter derived from an Agrobacterium tumefaciens opine synthase
gene, wherein at least one of said upstream activating elements are derived from
a different opine synthase gene than said promoter.
|
Claim 12
A cassette for inducible expression of a foreign gene in plants, said
cassette comprising said foreign gene operably linked to a
regulatory region comprising
a promoter derived from
a mannopine synthase gene of Agrobacterium tumefaciens by deletion to
base pair position -138 and
an upstream activating sequence derived
from a mannopine synthase gene of Agrobacterium tumefaciens.
|
Claim 13
A cassette for inducible expression of a foreign gene in plants, said
cassette comprising said foreign gene operably linked to a
regulatory region comprising
a promoter derived from
a mannopine synthase gene of Agrobacterium tumefaciens,
an
upstream activating sequence derived from a mannopine synthase gene of
Agrobacterium tumefaciens, and
an upstream activating
sequence derived from an octopine synthase gene of Agrobacterium
tumefaciens.
|
Claim 14
A cassette for expressing a gene in plants, said cassette
comprising a gene operably linked to a chimeric regulatory
region comprising at least three upstream activating sequences
derived from Agrobacterium tumefaciens octopine synthase genes operably
linked to a promoter derived from an Agrobacterium tumefaciens
mannopine synthase gene.
|
Claim 16
A method for expressing a gene in a plant, comprising the
steps of:
linking said gene to a chimeric regulatory region
comprising an upstream activating sequence derived from an
Agrobacterium tumefaciens octopine synthase gene operably linked to a
promoter derived from an Agrobacterium tumefaciens mannopine synthase
gene;
inserting said gene and said chimeric regulatory region into a
plant; and
allowing said plant to express said gene.
|
Claim 17
A method for expressing a gene in a plant, comprising the
steps of:
linking said gene to a chimeric regulatory region
comprising at least two upstream activating sequences derived
from an Agrobacterium tumefaciens opine synthase gene operably linked
to a promoter derived from an Agrobacterium tumefaciens opine synthase
gene, wherein at least one of said upstream activating elements are derived from
a different opine synthase gene than said promoter;
inserting said gene
and said chimeric regulatory region into a plant; and
allowing said
plant to express said gene.
|
Claim 21
A method for inducible expression of a foreign gene in a plant,
comprising:
linking said foreign gene to a regulatory region
comprising a promoter derived from a mannopine synthase gene of
Agrobacterium tumefaciens by deletion to base pair position -138 and an
upstream activating sequence derived from a mannopine synthase gene of
Agrobacterium tumefaciens;
inserting said foreign gene and
said regulatory region in said plant; and
inducing expression of said
foreign gene.
|
Claim 22
A method for inducible expression of a foreign gene in a plant,
comprising:
linking said foreign gene to a regulatory region
comprising
a promoter derived from a mannopine
synthase gene of Agrobacterium tumefaciens,
an upstream
activating sequence derived from a mannopine synthase gene of Agrobacterium
tumefaciens, and
an upstream activating sequence derived from
an octopine synthase gene of Agrobacterium tumefaciens;
inserting said foreign gene and said regulatory region in said plant; and
inducing expression of said foreign gene.
|
|
Claim 24
A method of expressing a gene in a plant, comprising the
steps of:
linking said gene to a chimeric regulatory region
comprising at least three upstream activating sequences derived
from an Agrobacterium tumefaciens octopine synthase gene operably
linked to a promoter derived from an Agrobacterium tumefaciens
mannopine synthase;
inserting said gene and said chimeric regulatory
region into a plant; and
allowing said plant to express said gene.
|
This Canadian patent claims an additional method of expressing a gene in a
plant using a chimeric regulatory region with at least 3 UAS from ocs gene and a
promoter from a mas gene.
|
There are no comments.