Patent Number
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Title, Independent Claims and Summary of Claims
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Assignee
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US
5352605
- Earliest priority - 17 January 1983
- Filed - 28 October 1993
- Granted - 4 October 1994
- Expected expiry - 4 October 2011
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Title - Chimeric genes for transforming plant cells using
viral promoters
Claim 1
A chimeric gene which is expressed in plant cells comprising a promoter from
a cauliflower mosaic virus, said promoter selected from the group consisting of
a CaMV (35S) promoter isolated from CaMV protein-encoding DNA sequences and a
CaMV (19S) promoter isolated from CaMV protein-encoding DNA sequences, and a
structural sequence which is heterologous with respect to the promoter.
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Claim 4
A plant cell which comprises a chimeric gene that contains a promoter from
cauliflower mosaic virus, said promoter selected from the group consisting of a
CaMV (35S) promoter and a CaMV (19S) promoter, wherein said promoter is isolated
from CaMV protein-encoding DNA sequences, and a structural sequence which is
heterologous with respect to the promoter.
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Claim 7
An intermediate plant transformation plasmid which comprises a region of
homology to an Agrobacterium tumefaciens vector, a T-DNA border region
from Agrobacterium tumefaciens and a chimeric gene, wherein the
chimeric gene is located between the T-DNA border and the region of homology,
said chimeric gene comprising a promoter from cauliflower mosaic virus, said
promoter selected from the group consisting of a CaMV(35S) promoter and a
CaMV(19S) promoter, and a structural sequence which is heterologous with respect
to the promoter.
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Claim 8
A plant transformation vector which comprises a disarmed plant tumor inducing
plasmid of Agrobacterium tumefaciens and a chimeric gene, wherein the
chimeric gene contains a promoter from cauliflower mosaic virus, said promoter
selected from the group consisting of a CaMV(35S) promoter and a CaMV(19S)
promoter, and a structural sequence which is heterologous with respect to the
promoter.
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Claim 13
A DNA construct comprising: (A) a CaMV promoter selected from the group
consisting of (1) a CaMV 35S promoter isolated from CaMV protein-encoding DNA
sequences and (2) a CaMV 19S promoter isolated from CaMV protein-encoding DNA
sequences, and (B) a DNA sequence of interest heterologous to (A), wherein (B)
is under the regulatory control of (A) when said construct is transcribed in a
plant cell.
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Claim 14
A chimeric gene which is transcribed and translated in plant cells, said
chimeric gene comprising a promoter from cauliflower mosaic virus, said promoter
selected from the group consisting of: a) a CaMV 35S promoter region free of
CaMV protein-encoding DNA sequences and b) a CaMV 19S promoter region free of
CaMV protein-encoding DNA sequences, and a DNA sequence which is heterologous
with respect to the promoter.
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Claim 15
A chimeric gene which is expressed in plants cells comprising a promoter from
a cauliflower mosaic virus, said promoter selected from the group consisting of
a CaMV(35S) promoter region free of CaMV protein-encoding DNA sequences and a
CaMV(19S) promoter region free of CaMV protein-encoding DNA sequences, and a DNA
sequence which is heterologous with respect to the promoter.
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Claim 16
A chimeric gene which is transcribed in plants cells comprising a promoter
from a cauliflower mosaic virus, said promoter selected from the group
consisting of a CaMV(35S) promoter free of CaMV protein-encoding DNA sequences
and a CaMV(19S) promoter free of CaMV protein-encoding DNA sequences, a DNA
sequence which is heterologous with respect to the promoter and a 3'
non-translated polyadenylation signal sequence.
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Claim 17
A plant cell which comprises a chimeric gene where said chimeric gene
comprises a promoter from cauliflower mosaic virus, said promoter selected from
the group consisting of a CaMV(35S) promoter and a CaMV(19S) promoter, wherein
said promoter is free of CaMV protein-encoding DNA sequences, and a DNA sequence
which is heterologous with respect to the promoter and a 3' non-translated
polyadenylation signal sequence.
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Claim elements:
- 35S and 19S promoters, as defined in the specification, are
claimed as part of a chimeric construct, also containing a heterologous signal
and a poly(A) signal, expressed in a plant
- There are also claims to intermediate plant transformation vectors and plant
transformation vectors containing the chimeric gene for
Agrobacterium-mediated plant transformation, and to plant cells having
the chimeric gene.
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Monsanto |
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US
5530196
- Earliest priority - 17 January 1983
- Filed - 2 September 1994
- Granted - 25 June 1996
- Expected expiry - 25 June 2013
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Title - Chimeric genes for transforming plant cells using
viral promoters
Claim 1
A differentiated dicotyledonous plant comprising plant cells containing a
chimeric gene which comprises a promoter from cauliflower mosaic virus, said
promoter selected from the group consisting of a CaMV(35S) promoter free of CaMV
protein-encoding DNA sequences and a CaMV(19S) promoter free of protein-encoding
DNA sequences, and a structural sequence which is heterologous with respect to
the promoter.
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Claim 4
A differentiated dicotyledonous plant comprising plant cells containing in
the plant genome a chimeric gene which comprises a promoter from cauliflower
mosaic virus, said promoter selected from the group consisting of a CaMV(35S)
promoter and a CaMV(19S) promoter, and a DNA sequence which is heterologous with
respect to the promoter.
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Claim 5
A differentiated dicotyledonous plant regenerated from plant cells, said
plant cells containing a chimeric gene which comprises a promoter from
cauliflower mosaic virus, said promoter selected from the group consisting of a
CaMV(35S) promoter and a CaMV(19S) promoter, and a DNA sequence which is
heterologous with respect to the promoter.
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This patent is a Continuation of
US
5352605.
- Differentiated dicot plants having plant cells with a
chimeric gene similar as described above. The poly(A) signal is not expressly
claimed as a component of the chimeric gene.
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US
5858742
- Earliest priority - 17 January 1983
- Filed - 24 June 1996
- Granted - 12 January 1999
- Expected expiry - 25 June 2013
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Title - Chimeric genes for transforming plant cells using
viral promoters
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Claim 1
A method for transforming a plant cell which
comprises transforming a plant cell with a chimeric DNA construct containing a
promoter isolated from cauliflower mosaic virus (CaMV), said promoter selected
from the group consisting of a CaMV(19S) promoter derived from the CaMV(19S)
gene and a CaMV(35S) promoter derived from the CaMV(35S) gene, and a DNA
sequence which is heterologous with respect to the promoter; wherein the
promoter regulates the transcription of the DNA sequence.
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This patent is a Continuation of
US
5530196.
- Method for transforming plant cells with a chimeric DNA construct as
generally described in
US
5530196.
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US
6255560
- Earliest priority - 17 January 1983
- Filed - 11 January 1999
- Granted - 3 July 2001
- Expired - 3 August 2005
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Title - Chimeric genes for transforming plant cells using
viral promoters
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Claim 1
A chimeric gene which is expressed in plant
cells comprising a promoter from cauliflower mosaic virus (CaMV), wherein said
promoter is the CaMV(19S) promoter or the CaMV(35S) promoter, operably linked to
a DNA sequence which is heterologous with respect to the promoter, wherein: the
promoter regulates the transcription of the DNA sequence, and the DNA sequence
encodes a polypeptide conferring increased antibiotic resistance to a plant or
plant cell containing the DNA sequence relative to a wild-type plant or plant
cell.
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Claim 3
A plant cell comprising a chimeric gene which
comprises a promoter from cauliflower mosaic virus (CaMV), wherein said promoter
is the CaMV(19S) promoter or the CaMV(35S) promoter, operably linked to a DNA
sequence which is heterologous with respect to the promoter, wherein: the
promoter regulates the transcription of the DNA sequence, and the DNA sequence
encodes a polypeptide conferring increased antibiotic resistance to the plant
cell relative to a wild-type plant cell.
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Claim 6
An intermediate plant transformation plasmid
which comprises a region of homology to an Agrobacterium tumefaciens
vector, a T-DNA border from Agrobacterium tumefaciens, and a chimeric gene,
wherein the chimeric gene is located between the T-DNA border and the region of
homology, said chimeric gene comprising a promoter from cauliflower mosaic virus
(CaMV), wherein said promoter is the CaMV(19S) promoter or the CaMV(35S)
promoter, operably linked to a DNA sequence which is heterologous with respect
to the promoter, wherein: the promoter regulates the transcription of the DNA
sequence, and the DNA sequence encodes a polypeptide conferring increased
antibiotic resistance to a plant or plant cell containing the DNA sequence
relative to a wild-type plant or plant cell.
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Claim 9
A plant transformation vector which comprises a
modified plant tumor inducing plasmid of Agrobacterium tumefaciens
which is capable of inserting a chimeric gene into susceptible plant cells,
wherein the chimeric gene comprises a promoter from cauliflower mosaic virus
(CaMV), wherein said promoter is the CaMV(19S) promoter or the CaMV(35S)
promoter, operably linked to a DNA sequence which is heterologous with respect
to the promoter, wherein: the promoter regulates the transcription of the DNA
sequence, and the DNA sequence encodes a polypeptide conferring increased
antibiotic resistance to a plant or plant cell containing the DNA sequence
relative to a wild-type plant or plant cell.
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Claim 12
A differentiated dicotyledonous plant
comprising plant cells containing a chimeric gene which comprises a promoter
from cauliflower mosaic virus (CaMV), wherein said promoter is the CaMV(19S)
promoter or the CaMV(35S) promoter, operably linked to a DNA sequence encoding
said polypeptide which is heterologous with respect to the promoter, wherein:
the promoter regulates the transcription of the DNA sequence, and the DNA
sequence encodes a polypeptide conferring increased antibiotic resistance to the
plant relative to a wild-type plant.
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This patent is a Continuation of
US
5858742.
- A chimeric gene where the 35S or the 19S promoter regulates the
transcription of a gene conferring antibiotic resistance to a plant.
- An intermediate plant transformation vector with the mentioned chimeric gene
between a T-DNA border and a region of homology to an Agrobacterium tumefaciens
vector.
- A plant transformation vector capable of inserting the chimeric gene into a
plant cells. Differentiated dicot plants having plant cells with the chimeric
gene.
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EP
131623 B2
- Earliest priority - 17 January 1983
- Filed - 16 January 1984
- Granted - 28 July 1999
- Lapsed after termination of 20 years
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Title - Chimeric genes suitable for expression in plant
cells
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Claim1
A chimeric gene capable of expressing a neomycin
phospotransferase polypeptide in plant cells conferring antibiotic resistance to
the plant when inserted into the plant genome, comprising in sequence:
a) a promoter region from a ribulose-1,5-bis-phosphate carboxyase small
subunit gene;
b) a 5' non-translated region
c) a structural coding
sequence encoding neomycin phospotransferase I or II; and
d) a 3'
non-translated region of a gene naturally expressed in plant cells, said region
encoding a signal sequence for polyadenylation of mRNA, said promoter region
being heterologous with respect to the structural coding sequence .
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Claim 4
A chimeric gene capable of expressing a
polypeptide in plant cells comprising in sequence:
a) a full-length transcript promoter region isolated from cauliflower mosaic
virus
b) a 5' non-translated region
c) a structural coding
sequence
d) a 3' non-translated region of a gene naturally expressed in
plants, said region encoding a signal sequence for polyadenylation of mRNA, said
structural coding sequence being heterologous with respect to said promoter
region.
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Claim 6
A culture of microorganisms identified by ATCC
accession number 39265.
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The claims are to:
- A chimeric gene capable of expressing a polypeptide in plant cells having a
35S promoter, an intron, a structural coding sequence and a
poly(A) signal.
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Remarks
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Granted Japanese patent JP 2645217 was not analyzed. There
are also a pending Japanese application (JP 7014349 B4) and a
pending Brazilian application (BR 1101069).
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